Part:BBa_J3901:Experience
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UNIQ03f503ac17180c27-partinfo-00000000-QINU UNIQ03f503ac17180c27-partinfo-00000001-QINU
This biobrick was sequenced by the Wageningen UR 2012 team. The sequencing result showed that this biobrick is lacking its iron inducable promotor.
Sequencing result:
>J3901 sequence from BaseClear:
GATTACTATAAATAGGCGTATCACGAGGCAGAATTTCGATAAAAAAATCC
TTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGCA
ATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTG
GCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTA
ATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTT
CCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACATA
CTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACGTTATCAA
AGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACG
AGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAG
ACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGA
CATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACC
CGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAA
TGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCA
GGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTG
GTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGT
TGGGAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGG
TGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTG
AAGTTAAAACCACCTACATGGCTAAAAACCGGTTCAGCTGGCGGGTGCTT
ACAAAACCGACATCAAACTGGACATCACCTCCCACAACGA
XbaI cutting site: TCTAGA
promoter (lacI regulated):
>BBa_R0010 Part-only sequence (200 bp)
CAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTG
GCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTA
ATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTT
CCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACA
As can be seen, the lacI regulated promotor starts right after the XbaI cutting site (1 bp between them), meaning the iron inducable promotor is lacking completely.